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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunoprecipitation [1]
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- Product number
- GTX631993 - Provider product page
- Provider
- GeneTex
- Product name
- c-Met antibody [GT1586]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT1586] (GTX631993) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- c-Met antibody detects c-Met protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody (GTX631993) diluted by 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and c-Met knockout (KO) HeLa cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT1586] (GTX631993) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT1586] (GTX631993) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and c-Met knockout (KO) HeLa cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT1586] (GTX631993) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of c-Met protein from HeLa whole cell extracts using 5 £gg of c-Met antibody [GT1586] (GTX631993).Western blot analysis was performed using c-Met antibody [GT1586] (GTX631993).EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.
