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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- TA347246 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal MeCP2 Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal MeCP2 Antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- MECP2
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µl
- Concentration
- not determined
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- C15310106-1,WB using the antibody against mouse MeCP2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- C15310106-2,Determination of the titer. To determine the titer, an ELISA was performed using a serial dilution of the antibody against mouse MeCP2. The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:136,000.
- Validation comment
- ELISA
