PA5-19748

antibody from Invitrogen Antibodies

Targeting: SMAD1 JV4-1, MADH1, MADR1

Antibody data

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Validation data

Product number: PA5-19748 - Provider product page
Provider: Invitrogen Antibodies
Product name: Anti-SMAD1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: For Western Blot, this antibody has non-specific bands at 27 kDa. This antibody is predicted to react with rat, sheep, horse, chicken, cow, human, pig, Xenopus laevis, chimpanzee and gorilla based on sequence homology.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 100 µg
Concentration: 0.6 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

SMAD1 protein structure - PA5-19748 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of HEK293 Whole Cell Lysate using Product # PA5-19748, Smad1 primary antibody at a dilution of 1 µg/mL. Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of SMAD1 was achieved by transfecting T-47D with SMAD1 specific siRNAs (Silencer® select Product # s8395, s8394). Western blot analysis (Fig. a) was performed using whole cell extracts from the SMAD1 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with SMAD1 Polyclonal Antibody (Product # PA5-19748, 1µg/ml) and chemiluminescence Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) . Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to SMAD1.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-SMAD1 Polyclonal Antibody (Product # PA5-19748) and a 55 kDa band corresponding to SMAD1 was observed along with uncharacterized bands at ~40 kDa across cell lines tested. Whole cell extracts (1% SDS) (30 µg lysate) of T-47D (Lane 1), MDA-MB-231 (Lane 2), OVCAR3 (Lane 3), HeLa (Lane 4), HCT 116 (Lane 5) and Ramos (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1µg/ml) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).