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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Flow cytometry [1]
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- Product number
- 17-5787-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NOTCH3 Monoclonal Antibody (MHN3-21), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This MNH3-21 monoclonal antibody reacts with the extracellular domain of human Notch3, one of four members of the Notch family of receptors. Notch receptors are 300-kDa single-pass transmembrane proteins. While the extracellular domain contains numerous epidermal growth factor-like repeats for ligand binding, the intracellular domain is involved in cell signaling. Upon binding its membrane-bound ligand (either Delta or Jagged), the Notch receptor undergoes proteolytic cleavage, first by ADAM-family metalloproteases and then by gamma-secretase. The second cleavage event releases the Notch intracellular domain (NICD), which subsequently translocates into the nucleus, heterodimerizes with the DNA-binding protein RBP-J, recruits co-activator molecules, and ultimately activates transcription.
- Antibody clone number
- MHN3-21
- Concentration
- 5 µL/Test
Submitted references Comprehensive Cell Surface Antigen Analysis Identifies Transferrin Receptor Protein-1 (CD71) as a Negative Selection Marker for Human Neuronal Cells.
Notch activation induces the generation of functional NK cells from human cord blood CD34-positive cells devoid of IL-15.
CADASIL: a critical look at a Notch disease.
Cross talk among Notch3, pre-TCR, and Tal1 in T-cell development and leukemogenesis.
Combined expression of pTalpha and Notch3 in T cell leukemia identifies the requirement of preTCR for leukemogenesis.
Menon V, Thomas R, Elgueta C, Horl M, Osborn T, Hallett PJ, Bartos M, Isacson O, Pruszak J
Stem cells (Dayton, Ohio) 2019 Oct;37(10):1293-1306
Stem cells (Dayton, Ohio) 2019 Oct;37(10):1293-1306
Notch activation induces the generation of functional NK cells from human cord blood CD34-positive cells devoid of IL-15.
Haraguchi K, Suzuki T, Koyama N, Kumano K, Nakahara F, Matsumoto A, Yokoyama Y, Sakata-Yanagimoto M, Masuda S, Takahashi T, Kamijo A, Takahashi K, Takanashi M, Okuyama Y, Yasutomo K, Sakano S, Yagita H, Kurokawa M, Ogawa S, Chiba S
Journal of immunology (Baltimore, Md. : 1950) 2009 May 15;182(10):6168-78
Journal of immunology (Baltimore, Md. : 1950) 2009 May 15;182(10):6168-78
CADASIL: a critical look at a Notch disease.
Louvi A, Arboleda-Velasquez JF, Artavanis-Tsakonas S
Developmental neuroscience 2006;28(1-2):5-12
Developmental neuroscience 2006;28(1-2):5-12
Cross talk among Notch3, pre-TCR, and Tal1 in T-cell development and leukemogenesis.
Talora C, Cialfi S, Oliviero C, Palermo R, Pascucci M, Frati L, Vacca A, Gulino A, Screpanti I
Blood 2006 Apr 15;107(8):3313-20
Blood 2006 Apr 15;107(8):3313-20
Combined expression of pTalpha and Notch3 in T cell leukemia identifies the requirement of preTCR for leukemogenesis.
Bellavia D, Campese AF, Checquolo S, Balestri A, Biondi A, Cazzaniga G, Lendahl U, Fehling HJ, Hayday AC, Frati L, von Boehmer H, Gulino A, Screpanti I
Proceedings of the National Academy of Sciences of the United States of America 2002 Mar 19;99(6):3788-93
Proceedings of the National Academy of Sciences of the United States of America 2002 Mar 19;99(6):3788-93
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of Jurkat cells with Mouse IgG1 kappa Isotype Control APC (Product # 17-4714-81) (blue histogram) or Anti-Human Notch3 APC (purple histogram). Total viable cells were used for analysis.
