711007

antibody from Invitrogen Antibodies

Targeting: NOTCH3 CADASIL, CASIL

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

711007

Provider

Invitrogen Antibodies

Product name

NOTCH3 Recombinant Polyclonal Antibody (4HCLC)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with Monkey, Bovine, Cat and Rat.

Antibody clone number

4HCLC

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of MCF7 (Lane 1), U-87 MG (Lane 2), SH-SY5Y (Lane 3), NTERA-2 (Lane 4), MDA-MB-231 (Lane 5), T-47D (Lane 6), U-2 OS (Lane 7) and A-431 (Lane 8). The blots were probed with Anti-Notch 3 Recombinant Rabbit Polyclonal Antibody (Product # 711007, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 110 kDa band corresponding to Notch 3 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with overnight wet transfer system. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).