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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- BML-PW0755-0025 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#BML-PW0755-0025, RRID:AB_2051890
- Product name
- Mono- and polyubiquitinylated conjugates monoclonal antibody (FK2) (biotin conjugate)
- Antibody type
- Monoclonal
- Antigen
- Purified from natural sources
- Description
- Ion exchange purified.
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- FK2
- Vial size
- 25 μl
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20¡C.
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Supportive validation
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- Enzo Life Sciences (provider)
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- Experimental details
- Western blot of K48-linked polyubiquitin chains (Ub2-7, Prod. No. BML-UW8860, 500ng) and K63-linked polyubiquitin chains (Ub2-7, Prod. No. BML-UW9570, 500ng) probed with MAb to Mono- and Polyubiquitinated ConjugatesÊ(FK2) (Biotin) (Prod. No. BML-PW0755) (1:1000 dilution), developed with streptavidin-HRP (Vectastain Elite ABC Kit, Vector Labs) and ECL Western blotting detection reagents.
