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- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Immunocytochemistry [3]
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- Product number
- BML-PW0600-0100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#BML-PW0600-0100, RRID:AB_11180533
- Product name
- Polyubiquitin (K63-linkage-specific) monoclonal antibody (HWA4C4)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- HWA4C4
- Vial size
- 100 μl
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20¡C.
Submitted references CYLD and the NEMO Zinc Finger Regulate Tumor Necrosis Factor Signaling and Early Embryogenesis.
Cytosolic PTEN-induced Putative Kinase 1 Is Stabilized by the NF-κB Pathway and Promotes Non-selective Mitophagy.
Pale body-like inclusion formation and neurodegeneration following depletion of 26S proteasomes in mouse brain neurones are independent of α-synuclein.
Zhao Y, Ma CA, Wu L, Iwai K, Ashwell JD, Oltz EM, Ballard DW, Jain A
The Journal of biological chemistry 2015 Sep 4;290(36):22076-84
The Journal of biological chemistry 2015 Sep 4;290(36):22076-84
Cytosolic PTEN-induced Putative Kinase 1 Is Stabilized by the NF-κB Pathway and Promotes Non-selective Mitophagy.
Lim GG, Chua DS, Basil AH, Chan HY, Chai C, Arumugam T, Lim KL
The Journal of biological chemistry 2015 Jul 3;290(27):16882-93
The Journal of biological chemistry 2015 Jul 3;290(27):16882-93
Pale body-like inclusion formation and neurodegeneration following depletion of 26S proteasomes in mouse brain neurones are independent of α-synuclein.
Paine SM, Anderson G, Bedford K, Lawler K, Mayer RJ, Lowe J, Bedford L
PloS one 2013;8(1):e54711
PloS one 2013;8(1):e54711
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot of single isopeptide-linkage polyubiquitinylated conjugates K6 (BML-UW0615), K11 (BML-UW0620), K27 (BML-UW0625), K29 (BML-UW0630), K33 (BML-UW0635), K48 (BML-UW0640), K63 (BML-UW0645),1µg each, using MAb to Polyubiquitinylated Conjugates (FK1) (BML-PW8805) and MAb to Polyubiquitin (K63-linkage-specific) (HWA4C4) (BML-PW0600)Êat 1:1'000 dilution.
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot of HeLa S100 Fraction (BML-SW8750, 5µg) probed withÊMAb to Polyubiquitin (K63-linkage-specific) (HWA4C4) (BML-PW0600) (Lane A)ÊandÊMAb to Polyubiquitinylated Conjugates (FK1) (BML-PW8805)Ê(Lane B)Êat 1:1'000 dilution.
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Formalin-fixed paraffin-embedded sections of hippocampus from human Alzheimer's disease brain stained withÊMAb to Polyubiquitin (K63-linkage-specific) (HWA4C4) at 1:100 dilution with biotinylated secondary antibody and DAB detection.
