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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [18]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AF643 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal VCAM-1/CD106 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse VCAM-1/CD106 in direct ELISAs and Western blots. In direct ELISA and Western blots, approximately 5% cross-reactivity with recombinant human VCAM-1 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Intermittent Hypoxia Activates Duration-Dependent Protective and Injurious Mechanisms in Mouse Lung Endothelial Cells.
Differential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus.
Neutrophil infiltration to the brain is platelet-dependent, and is reversed by blockade of platelet GPIbα.
Endothelium-derived extracellular vesicles promote splenic monocyte mobilization in myocardial infarction.
Suberanilohydroxamic Acid as a Pharmacological Kruppel-Like Factor 2 Activator That Represses Vascular Inflammation and Atherosclerosis.
Loss of Ptpn11 (Shp2) drives satellite cells into quiescence.
Numb regulates somatic cell lineage commitment during early gonadogenesis in mice.
High soluble endoglin levels do not induce endothelial dysfunction in mouse aorta.
Platelet-driven leukotriene C4-mediated airway inflammation in mice is aspirin-sensitive and depends on T prostanoid receptors.
Control of the T follicular helper-germinal center B-cell axis by CD8⁺ regulatory T cells limits atherosclerosis and tertiary lymphoid organ development.
Icam-1 targeted nanogels loaded with dexamethasone alleviate pulmonary inflammation.
CD44-deficiency attenuates the immunologic responses to LPS and delays the onset of endotoxic shock-induced renal inflammation and dysfunction.
Targeting improves MSC treatment of inflammatory bowel disease.
Mst1 controls lymphocyte trafficking and interstitial motility within lymph nodes.
Dietary alpha-lipoic acid supplementation inhibits atherosclerotic lesion development in apolipoprotein E-deficient and apolipoprotein E/low-density lipoprotein receptor-deficient mice.
Osteoblasts support B-lymphocyte commitment and differentiation from hematopoietic stem cells.
Mice lacking NKCC1 are protected from development of bacteremia and hypothermic sepsis secondary to bacterial pneumonia.
High pressure promotes monocyte adhesion to the vascular wall.
Wohlrab P, Soto-Gonzales L, Benesch T, Winter MP, Lang IM, Markstaller K, Tretter V, Klein KU
Frontiers in physiology 2018;9:1754
Frontiers in physiology 2018;9:1754
Differential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus.
Moretti FA, Klapproth S, Ruppert R, Margraf A, Weber J, Pick R, Scheiermann C, Sperandio M, Fässler R, Moser M
eLife 2018 Sep 6;7
eLife 2018 Sep 6;7
Neutrophil infiltration to the brain is platelet-dependent, and is reversed by blockade of platelet GPIbα.
Giles JA, Greenhalgh AD, Denes A, Nieswandt B, Coutts G, McColl BW, Allan SM
Immunology 2018 Jun;154(2):322-328
Immunology 2018 Jun;154(2):322-328
Endothelium-derived extracellular vesicles promote splenic monocyte mobilization in myocardial infarction.
Akbar N, Digby JE, Cahill TJ, Tavare AN, Corbin AL, Saluja S, Dawkins S, Edgar L, Rawlings N, Ziberna K, McNeill E, Oxford Acute Myocardial Infarction (OxAMI) Study, Johnson E, Aljabali AA, Dragovic RA, Rohling M, Belgard TG, Udalova IA, Greaves DR, Channon KM, Riley PR, Anthony DC, Choudhury RP
JCI insight 2017 Sep 7;2(17)
JCI insight 2017 Sep 7;2(17)
Suberanilohydroxamic Acid as a Pharmacological Kruppel-Like Factor 2 Activator That Represses Vascular Inflammation and Atherosclerosis.
Xu Y, Xu S, Liu P, Koroleva M, Zhang S, Si S, Jin ZG
Journal of the American Heart Association 2017 Nov 30;6(12)
Journal of the American Heart Association 2017 Nov 30;6(12)
Loss of Ptpn11 (Shp2) drives satellite cells into quiescence.
Griger J, Schneider R, Lahmann I, Schöwel V, Keller C, Spuler S, Nazare M, Birchmeier C
eLife 2017 May 2;6
eLife 2017 May 2;6
Numb regulates somatic cell lineage commitment during early gonadogenesis in mice.
Lin YT, Barske L, DeFalco T, Capel B
Development (Cambridge, England) 2017 May 1;144(9):1607-1618
Development (Cambridge, England) 2017 May 1;144(9):1607-1618
High soluble endoglin levels do not induce endothelial dysfunction in mouse aorta.
Nemeckova I, Serwadczak A, Oujo B, Jezkova K, Rathouska J, Fikrova P, Varejckova M, Bernabeu C, Lopez-Novoa JM, Chlopicki S, Nachtigal P
PloS one 2015;10(3):e0119665
PloS one 2015;10(3):e0119665
Platelet-driven leukotriene C4-mediated airway inflammation in mice is aspirin-sensitive and depends on T prostanoid receptors.
Liu T, Garofalo D, Feng C, Lai J, Katz H, Laidlaw TM, Boyce JA
Journal of immunology (Baltimore, Md. : 1950) 2015 Jun 1;194(11):5061-8
Journal of immunology (Baltimore, Md. : 1950) 2015 Jun 1;194(11):5061-8
Control of the T follicular helper-germinal center B-cell axis by CD8⁺ regulatory T cells limits atherosclerosis and tertiary lymphoid organ development.
Clement M, Guedj K, Andreata F, Morvan M, Bey L, Khallou-Laschet J, Gaston AT, Delbosc S, Alsac JM, Bruneval P, Deschildre C, Le Borgne M, Castier Y, Kim HJ, Cantor H, Michel JB, Caligiuri G, Nicoletti A
Circulation 2015 Feb 10;131(6):560-70
Circulation 2015 Feb 10;131(6):560-70
Icam-1 targeted nanogels loaded with dexamethasone alleviate pulmonary inflammation.
Ferrer MC, Shuvaev VV, Zern BJ, Composto RJ, Muzykantov VR, Eckmann DM
PloS one 2014;9(7):e102329
PloS one 2014;9(7):e102329
CD44-deficiency attenuates the immunologic responses to LPS and delays the onset of endotoxic shock-induced renal inflammation and dysfunction.
Rampanelli E, Dessing MC, Claessen N, Teske GJ, Joosten SP, Pals ST, Leemans JC, Florquin S
PloS one 2013;8(12):e84479
PloS one 2013;8(12):e84479
Targeting improves MSC treatment of inflammatory bowel disease.
Ko IK, Kim BG, Awadallah A, Mikulan J, Lin P, Letterio JJ, Dennis JE
Molecular therapy : the journal of the American Society of Gene Therapy 2010 Jul;18(7):1365-72
Molecular therapy : the journal of the American Society of Gene Therapy 2010 Jul;18(7):1365-72
Mst1 controls lymphocyte trafficking and interstitial motility within lymph nodes.
Katagiri K, Katakai T, Ebisuno Y, Ueda Y, Okada T, Kinashi T
The EMBO journal 2009 May 6;28(9):1319-31
The EMBO journal 2009 May 6;28(9):1319-31
Dietary alpha-lipoic acid supplementation inhibits atherosclerotic lesion development in apolipoprotein E-deficient and apolipoprotein E/low-density lipoprotein receptor-deficient mice.
Zhang WJ, Bird KE, McMillen TS, LeBoeuf RC, Hagen TM, Frei B
Circulation 2008 Jan 22;117(3):421-8
Circulation 2008 Jan 22;117(3):421-8
Osteoblasts support B-lymphocyte commitment and differentiation from hematopoietic stem cells.
Zhu J, Garrett R, Jung Y, Zhang Y, Kim N, Wang J, Joe GJ, Hexner E, Choi Y, Taichman RS, Emerson SG
Blood 2007 May 1;109(9):3706-12
Blood 2007 May 1;109(9):3706-12
Mice lacking NKCC1 are protected from development of bacteremia and hypothermic sepsis secondary to bacterial pneumonia.
Nguyen M, Pace AJ, Koller BH
The Journal of experimental medicine 2007 Jun 11;204(6):1383-93
The Journal of experimental medicine 2007 Jun 11;204(6):1383-93
High pressure promotes monocyte adhesion to the vascular wall.
Riou S, Mees B, Esposito B, Merval R, Vilar J, Stengel D, Ninio E, van Haperen R, de Crom R, Tedgui A, Lehoux S
Circulation research 2007 Apr 27;100(8):1226-33
Circulation research 2007 Apr 27;100(8):1226-33
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse VCAM-1/CD106 by Western Blot. Western blot shows lysates of 3T3-L1 mouse embryonic fibroblast adipose-like cell line and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse VCAM-1/CD106 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF643) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for VCAM-1/CD106 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse VCAM-1/CD106 by Simple WesternTM. Simple Western lane view shows lysates of C2C12 mouse myoblast cell line and 3T3-L1 mouse embryonic fibroblast adipose-like cell line, loaded at 0.2 mg/mL. A specific band was detected for VCAM-1/CD106 at approximately 116 kDa (as indicated) using 1 µg/mL of Goat Anti-Mouse VCAM-1/CD106 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF643) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- VCAM-1/CD106 in Mouse Embryo. VCAM-1/CD106 was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Mouse VCAM-1/CD106 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF643) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of VCAM-1/CD106 in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with Goat Anti-Mouse VCAM-1/CD106 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF643, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
