Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- NB100-664AF488 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal VEGF Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. This VEGF Antibody (VG1) detects the 189, 165 and 121 isoforms of VEGF
- Reactivity
- Human, Mouse, Rat, Canine
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C in the dark.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Flow Cytometry: VEGF Antibody (VG1) [Alexa Fluor® 488] [NB100-664AF488] - An intracellular stain was performed on U-87 cells with VEGF [VG1] Antibody NB100-664AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.