MAB3045-100

antibody from R&D Systems

Targeting: VEGFA VEGF, VEGF-A, VPF

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB3045-100

Provider

R&D Systems

Product name

Human VEGF165b Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human VEGF165b in direct ELISAs and Western blots. Recognizes an epitope within a 9 amino acid sequence at the C-terminus of human VEGF165b. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) VEGF206, rhVEGF-B167, rhVEGF-B186, rhVEGF-C, or rhVEGF-D is observed.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

AAL27435


Isotype

IgG

Antibody clone number

56-1

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Bone marrow-derived cells are implicated as a source of lymphatic endothelial progenitors in human breast cancer.

Van't Hull EF, Bron S, Henry L, Ifticene-Treboux A, Turrini R, Coukos G, Delaloye JF, Doucey MA
Oncoimmunology 2014;3:e29080

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The role of VEGF-A165b in trophoblast survival.

Bills VL, Hamdollah-Zadeh M, Soothill PW, Harper SJ, Bates DO
BMC pregnancy and childbirth 2014 Aug 15;14:278

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Detection of VEGF-A(xxx)b isoforms in human tissues.

Bates DO, Mavrou A, Qiu Y, Carter JG, Hamdollah-Zadeh M, Barratt S, Gammons MV, Millar AB, Salmon AH, Oltean S, Harper SJ
PloS one 2013;8(7):e68399

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The transcription factor E2F1 and the SR protein SC35 control the ratio of pro-angiogenic versus antiangiogenic isoforms of vascular endothelial growth factor-A to inhibit neovascularization in vivo.

Merdzhanova G, Gout S, Keramidas M, Edmond V, Coll JL, Brambilla C, Brambilla E, Gazzeri S, Eymin B
Oncogene 2010 Sep 30;29(39):5392-403

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VEGF in the lung: a role for novel isoforms.

Varet J, Douglas SK, Gilmartin L, Medford AR, Bates DO, Harper SJ, Millar AB
American journal of physiology. Lung cellular and molecular physiology 2010 Jun;298(6):L768-74

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VEGF(121)b, a new member of the VEGF(xxx)b family of VEGF-A splice isoforms, inhibits neovascularisation and tumour growth in vivo.

Rennel ES, Varey AH, Churchill AJ, Wheatley ER, Stewart L, Mather S, Bates DO, Harper SJ
British journal of cancer 2009 Oct 6;101(7):1183-93

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The endogenous anti-angiogenic family of splice variants of VEGF, VEGFxxxb, are down-regulated in pre-eclamptic placentae at term.

Bates DO, MacMillan PP, Manjaly JG, Qiu Y, Hudson SJ, Bevan HS, Hunter AJ, Soothill PW, Read M, Donaldson LF, Harper SJ
Clinical science (London, England : 1979) 2006 May;110(5):575-85

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

VEGF_165b in Human Placenta. VEGF_165b was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human VEGF_165b Monoclonal Antibody (Catalog # MAB3045) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblasts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.