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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- IC2931V-100UG - Provider product page
- Provider
- R&D Systems
- Product name
- Human VEGF Alexa Fluor® 405-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human VEGF.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Blue dye
- Isotype
- IgG
- Antibody clone number
- 23410
- Vial size
- 100 ug
- Concentration
- 0.2 mg/ml
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of VEGF in U937 Human Cell Line by Flow Cytometry U937 human histiocytic lymphoma cell line either treated with 50 ng/mL PMA and 200 ng/mL Calcium Ionomycin for 16 hours (filled histogram) or untreated (open histogram) was stained with Mouse Anti-Human VEGF Alexa Fluor® 405-conjugated Monoclonal Antibody (Catalog # IC2931V). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with methanol. View our protocol for Staining Intracellular Molecules.
