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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- PA1-31775 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-gamma Adaptin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-31775 detects AP1G1 from human samples.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.7 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot using gamma Adaptin Polyclonal Antibody (Product # PA1-31775) shows strong detection of a 91-kDa band corresponding to Human AP1G1 in a HeLa whole cell lysate (lane 1). Peptide competition (using 1 µg/mL of the immunizing peptide) blocks the specific reactivity of this antibody with AP1G1 (lane 2). Approximately 20 µg of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP1G1 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A chemi-luminescence system was used for signal detection using a 60-sec exposure time.
