MA5-15917

antibody from Invitrogen Antibodies

Targeting: MSH6 GTBP

Antibody data

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Validation data

Product number: MA5-15917 - Provider product page
Provider: Invitrogen Antibodies
Product name: MSH6 Monoclonal Antibody (5B11)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: MA5-15917 targets MSH6 in WB applications and shows reactivity with Human samples.
Antibody clone number: 5B11
Concentration: Conc. Not Determined

MSH6 protein structure - MA5-15917 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-MSH6 Monoclonal Antibody (5B11) (Product # MA5-15917) and a 160kDa band corresponding to MSH6 was observed in all the tested cell models, except Jurkat and LNCaP which are reported to be negative. This target undergoes heavy phosphorylation and multiple bands between 120-160kDa can be expected. Modified whole cell lysate (1% SDS) (30ug lysate) of K-562 (Lane 1), Jurkat (Lane 2), HeLa (Lane 3), LNCaP (Lane 4), PC-3 (Lane 5), HCT 116 (Lane 6) and A549 (Lane 7) were electrophoresed using NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of MSH6 using a MSH6 monoclonal antibody (Product # MA5-15917) against a human MSH6 (AA: 217-395) recombinant protein.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of MSH6 using MSH6 monoclonal antibody (Product # MA5-15917) in MCF-7 (1), HEK293 (2), and HCT116 (3) cell lysate.