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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- PA5-85353 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MyD88 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Keep as concentrated solution.
- Concentration
- 0.93 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MyD88 in non-transfected (–) and transfected (+) 293T whole cell extracts using MyD88 polyclonal antibody (Product # PA5-85353) using 30 µg of sample at a dilution of 1:5000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 12% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using MyD88 Polyclonal Antibody (Product # PA5-85353). Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with MyD88 Polyclonal Antibody (Product # PA5-85353) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ELISA detection of MyD88 using MyD88 Polyclonal Antibody (Product # PA5-85353) for capture at a concentration of 5 µg/mL and a MyD88 Polyclonal Antibody for detection at a concentration of 5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MYD88 antibody detects MYD88 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min. Green: MYD88 protein stained by MYD88 antibody (Product # PA5-85353) diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
