PA5-37360

antibody from Invitrogen Antibodies

Targeting: DCTN1

Antibody data

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Validation data

Product number: PA5-37360 - Provider product page
Provider: Invitrogen Antibodies
Product name: Dynactin 1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: This antibody detects endogenous protein at a molecular weight of 150 kDa.
Concentration: 1 mg/mL

DCTN1 protein structure - PA5-37360 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Dynactin 1. Samples were incubated with Dynactin 1 polyclonal antibody (Product # PA5-37360).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of DCTN1 using DCTN1 polyclonal antibody (Product # PA5-37360).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of DCTN1 using DCTN1 polyclonal antibody (Product # PA5-37360).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of Dynactin 1 was achieved by transfecting HeLa with DCTN1 specific siRNAs (Silencer® select Product # s3973, s3974). Western blot analysis (Fig. a) was performed using whole cell extracts from the DCTN1 knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with Dynactin 1 Polyclonal Antibody (Product # PA5-37360, 1:1500 dilution) and Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to DCTN1.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-Dynactin 1 Polyclonal Antibody (Product # PA5-37360) and a 141 kDa band corresponding to DCTN1 was observed across all the cell lines tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), Jurkat (Lane 2), SH-SY5Y (Lane 3) and U-87 MG (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).