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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-22345 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SMAD9 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1.
- Concentration
- 0.68 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SMAD9 using 30 µg of NT2D1 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a SMAD9 polyclonal antibody (Product # PA5-22345) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SMAD9 Polyclonal Antibody (Product # PA5-22345). Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with SMAD9 Polyclonal Antibody (Product # PA5-22345) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SMAD9 in paraformaldehyde-fixed HeLa cells using a SMAD9 polyclonal antibody (Product # PA5-22345) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SMAD9 Polyclonal Antibody detects SMAD9 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. SMAD9 Polyclonal Antibody (Product # PA5-22345) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
