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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunohistochemistry [2]
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- Product number
- PA5-47950 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- XIAP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute at 0.2 mg/mL in sterile PBS.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of Jurkat human acute T cell leukemia cell line, C2C12 mouse myoblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-human/mouse/Rat XIAP Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47950) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for XIAP at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockout validation by Western blot analysis of XIAP in lysates of HeLa human cervical epithelial carcinoma parental cell line and XIAP knockout HeLa cell line (KO). Samples were incubated in XIAP polyclonal antibody (Product # PA5-47950) using a dilution of 0.5 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for XIAP at approximately 52 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockoutHeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of XIAP in Jurkat human acute T cell leukemia cell line, C2C12 mouse myoblast cell line, and PC‚12 rat adrenal pheochromocytoma cell line. Samples were incubated in XIAP polyclonal antibody (Product # PA5-47950) using a dilution of 0.5 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for XIAP at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of XIAP in 0.2 mg/mL lysates of Jurkat human acute T cell leukemia cell line and C2C12 mouse myoblast cell line. Samples were incubated in XIAP polyclonal antibody (Product # PA5-47950) using a dilution of 5 µg/mL followed by HRP-conjugated Anti-Goat IgG at a dilution of 0.0763888888888889. A specific band was detected for XIAP at approximately 59 kDa (as indicated). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of XIAP in immersion fixed paraffin-embedded sections of human lymphoma. Samples were incubated in XIAP polyclonal antibody (Product # PA5-47950) using a dilution of 5 µg/mL overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of XIAP in immersion fixed paraffin-embedded sections of human breast cancer tissue. Samples were incubated in XIAP polyclonal antibody (Product # PA5-47950) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).
