Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 687102 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#687102, RRID:AB_2616940
- Product name
- Purified anti-human Ikaros
- Antibody type
- Monoclonal
- Antigen
- Human Ikaros peptide (431-477 aa) conjugated to KLH Freund's adjuvant.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 16B5C71
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Total cell lysate from human Jurkat cells (lane 1), HeLa cells (lane 2), and Raji cells (lane 3) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-Ikaros (clone 16B5C71) antibody. Proteins were visualized using an HRP goat anti-mouse IgG antibody and chemiluminescence detection. Purified anti-Tubulin-α (clone XX) antibody was used as a loading control.
- Conjugate
- Unconjugated