Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [7]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX111156 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX111156, RRID:AB_10618250
- Product name
- Dishevelled 2 antibody [N1N3]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: HCT116 7.5% SDS PAGE GTX111156 diluted at 1:5000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Dishevelled 2 antibody [N1N3] (GTX111156) diluted at 1:10000 and competitor's antibody (SC-13974) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Dishevelled 2 antibody [N1N3] (GTX111156) diluted at 1:5000 and competitor's antibody (SC-13974) diluted by 1:200.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Dishevelled 2 antibody [N1N3] (GTX111156) diluted at 1:10000 and competitor's antibody (sc-13974) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dishevelled 2 antibody [N1N3] (GTX111156) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dishevelled 2 antibody [N1N3] (GTX111156) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Dishevelled 2 antibody [N1N3] (GTX111156) diluted at 1:10000 and competitor's antibody (sc-13974) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Dvl-2 antibody [N1N3] detects Dvl-2 protein at cytoplasm by immunofluorescent analysis.Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Dvl-2 protein stained by Dvl-2 antibody [N1N3] (GTX111156) diluted at 1:500.Blue: Hoechst 33342 staining.
