TA300956

antibody from OriGene

Targeting: YAP1 YAP65

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA300956

Provider

OriGene

Product name

Rabbit Monoclonal Antibody against YAP1 (Clone EP1674Y)

Antibody type

Monoclonal

Description

Rabbit Monoclonal Antibody against YAP1 (Clone EP1674Y)

Host

Rabbit

Conjugate

Unconjugated

Epitope

YAP1

Isotype

IgG

Antibody clone number

EP1674Y

Vial size

100 µl

Concentration

NULL

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot - YAP1 antibody [EP1674Y]; Anti-YAP1 antibody [EP1674Y] at 1/50000 dilution + Hela cell lysate at 10 ug.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 65 kDa.Observed band size : 65 kDa.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot - YAP1 antibody [EP1674Y]; .developed using the ECL technique.Predicted band size : 65 kDa.Observed band size : 70 kDa .Exposure time : 1 minute

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin-embedded sections) - YAP1 antibody [EP1674Y]; Immunohistochemical staining of YAP1 in paraffin embedded human breast carcinoma tissue using TA300956 at a 1/100 dilution.

Validation comment

IHC

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow Cytometry - YAP1 antibody; Overlay histogram showing HeLa cells stained with TA300956 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Validation comment

FC