21465-1

antibody from Novus Biologicals

Targeting: IFNB1 IFB, IFF, IFNB

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

21465-1

Provider

Novus Biologicals

Product name

Mouse Monoclonal IFN-beta Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Binds to human interferon beta with high affinity. The ND50 for this antibody on human (A549/EMCV) cells is ~27-66 µg/ml in the presence of 96 pg/ml of recombinant human interferon beta 1a, mammalian.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

50 ug

Storage

After receipt, the product may be stored at -20degreesC for short-term use (= 6 months). For long-term storage, we recommend storing the product at -70degreesC or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the product should be in buffers containing protein such as 0.1% bovine serum albumin (BSA).

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

ELISA: IFN-beta Antibody [21465-1] - Representative binding curve of antibody to mammalian expressed human interferon beta 1a (circle) and to 0.1% BSA/PBS (square) in a Direct Binding ELISA. Medium-binding polystyrene plates were coated with either 1 ug/ml human interferon beta 1a or 0.1% BSA/PBS. Thereafter, titrations of the product were added to the wells. Donkey anti-mouse IgG conjugated to HRP was used as the detection antibody. Colorimetric detection was performed using 3, 3', 5, 5'-Tetramethylbenzidine (TMB) substrate. The HRP-TMB reaction was stopped using a diluted H2SO4/HCl solution.