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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- TA347113 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal DNMT3A Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal DNMT3A Antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- DNMT3A
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 50 µg
- Concentration
- 0.93 ug/ul
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB analysis with anti-GAL4 antibody (lane 1 and 2 respectively). Alternatively, GAL4-DNMT3A was immunoprecipitated and WB analysis was performed with the DNMT3A antibody (diluted 1:2,500) (lane 3).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against human DNMT3A, crude serum and Flow Through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,000.
- Validation comment
- ELISA
