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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- TA347115 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal DNMT3A Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal DNMT3A Antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- DNMT3A
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µl
- Concentration
- not determined
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- C15310107-1,WB using the antibody against mouse DNMT3A diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- To determine the titer of the antibody, ELISA was performed using a serial dilution of ab against mouse DNMT3A. The wells were coated with the peptides used as antigen. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:86,500. Figure 2: WB using the ab against mouse DNMT3A at 1:500 in TBS-Tween containing 5% milk.
- Validation comment
- ELISA
