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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- PAB14129 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB14129, RRID:AB_10549310
- Product name
- DNMT3A polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of DNMT3A.
- Storage
- Store at -20°C. For long term storage store at -80°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Human embryonic kidney cells (HEK293T) were transiently transfected with an expression vector for GAL4-tagged DNMT3A.Whole cell extracts were immunoprecipitated with 2 ug of DNMT3A polyclonal antibody (Cat # PAB14129).The presence of GAL4-DNMT3A in the non-treated cell extracts and in the immunoprecipitates was demonstrated by western blot analysis with anti-GAL4 antibody (Lane 1 and 2 respectively).Alternatively, GAL4-DNMT3A was immunoprecipitated and western blot analysis was performed with the DNMT3A polyclonal antibody (Cat # PAB14129) (diluted 1 : 2,000) (Lane 3).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA was performed using a serial dilution of DNMT3A polyclonal antibody (Cat # PAB14129), crude serum and Flow Through, in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1 : 500.
- Validation comment
- ELISA
