STJ114766
antibody from St John's Laboratory
Targeting: ZBTB33
kaiso, WUGSC:H_DJ525N14.1, ZNF-kaiso, ZNF348
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Chromatin Immunoprecipitation [1]
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Validation data
Reference
Comment
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- Product number
- STJ114766 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-ZBTB33 antibody (523-672) (STJ114766)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody anti-ZBTB33 (523-672) is suitable for use in Western Blot.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
NA
- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store in a freezer at-20°C and avoid freeze-thaw cycles.
- Handling
- NA
No comments: Submit comment
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using Kaiso/Kaiso/ZBTB33 antibody (STJ114766) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Kaiso/ZBTB33 antibody (STJ114766) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA