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Immunocytochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- 644830 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#644830, RRID:AB_2566019
- Product name
- Alexa Fluor® 488 anti-T-bet
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- T-bet, also known as T-box transcription factor T-bet, is considered to be a "master regulator" of Th1 lymphoid development controlling the production of the cytokine IFN-γ. T-bet is widely expressed in hematopoietic cells including stem cells, NK cells, B cells, and T cells. T-bet is critical for the control of microbial pathogens, and knockout animals show multiple physiologic and inflammatory features characteristic of asthma. T-bet expression is optimally observed after IL-12 stimulation and can be suppressed by addition of the Th2 cytokine IL-4 or neutralization of IL-12.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Green dye
- Antibody clone number
- 4B10
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Human peripheral blood lymphocytes were surface stained with CD3 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with T-bet (clone 4B10) Alexa Fluor® 488 (top) or mouse IgG1, κ Alexa Fluor® 488 isotype control (bottom).
- Conjugate
- Green dye
