644832

antibody from BioLegend

Targeting: TBX21 T-bet, TBLYM

Immunocytochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

644832

Provider

BioLegend

Proper citation

BioLegend Cat#644832, RRID:AB_2686976

Product name

Brilliant Violet 421™ anti-T-bet

Antibody type

Monoclonal

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Blue dye

Antibody clone number

4B10

Vial size

50 µg

Concentration

0.2 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Human peripheral blood lymphocytes were surface stained with CD3 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set (Cat# 424401). Cells were then stained with T-bet (clone 4B10) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).

Conjugate

Blue dye