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Immunocytochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- 644815 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#644815, RRID:AB_10896427
- Product name
- Brilliant Violet 421™ anti-T-bet [4B10]
- Antibody type
- Monoclonal
- Antigen
- T-bet
- Description
- T-bet, also known as T-box transcription factor T-bet, is considered to be a "master regulator" of Th1 lymphoid development controlling the production of the cytokine IFN-γ. T-bet is widely expressed in hematopoietic cells including stem cells, NK cells, B cells, and T cells. T-bet is critical for the control of microbial pathogens, and knockout animals show multiple physiologic and inflammatory features characteristic of asthma. T-bet expression is optimally observed after IL-12 stimulation and can be suppressed by addition of the Th2 cytokine IL-4 or neutralization of IL-12.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Blue dye
- Isotype
- IgG
- Vial size
- 125 µl
- Storage
- The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- Human peripheral blood lymphocytes were surface stained with CD3 APC and then intracellularly stained with T-bet (clone 4B10) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ (bottom).
- Conjugate
- Blue dye
