PA1-27800

antibody from Invitrogen Antibodies

Targeting: ATP6V1E1 ATP6E, ATP6E2, ATP6V1E, P31, Vma4

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-27800

Provider

Invitrogen Antibodies

Product name

Anti-V-ATPase E1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA1-27800 detects ATP6V1E1 from human, mouse, and rat samples. PA1-27800 may be successfully used in Western blot applications. By Western blot, this antibody has only been tested on fusion protein. The immunogen is a synthetic peptide corresponding to human Vacuolar ATP synthase subunit E.

Reactivity

Human, Mouse, Rat

Host

Chicken/Avian

Isotype

IgY

Vial size

50 µg

Concentration

1 mg/ml

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ATP6V1E1 in lysate expressing protein V-type proton ATPase subunit E. Samples were probed with an ATP6V1E1 polyclonal antibody (Product # PA1-27800) at a dilution of 1:1000 dilution. Secondary detection was performed using a peroxidase conjugated Rabbit anti-Chicken IgY (IgG) at a 1:10,000 dilution and developed with TMB Substrate. Gene specific transient over-expression HEK293T cell lysate (lane 2); Empty vector transfected control HEK293T cell lysate (lane 3).