Explore
Validate
Learn
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX77645 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX77645, RRID:AB_424593
- Product name
- CENPF antibody [7F11.2]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
Submitted references Assessment of histologic features and expression of biomarkers in predicting pathologic response to anthracycline-based neoadjuvant chemotherapy in patients with breast carcinoma.
Wang J, Buchholz TA, Middleton LP, Allred DC, Tucker SL, Kuerer HM, Esteva FJ, Hortobagyi GN, Sahin AA
Cancer 2002 Jun 15;94(12):3107-14
Cancer 2002 Jun 15;94(12):3107-14
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescent staining of mitotic U2OS cells with mitosin/CENP-F antibody GTX77645 (green) at 1:500 dilution. Cells were pre-extracted with ice-cold CSK buffer (0.5% Triton X-100, 300 mM Sucrose, 10 mM PIPES, pH8.0) for 4 min, followed by fixation with 4% PFA and regular IFA procedure. Blue channel (DAPI staining) shows chromosomes.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- High magnification photomicrograph of an NG3 tumor with a high percentage of mitosin-positive nuclei, indicative of high proliferative activity.
