711584

antibody from Invitrogen Antibodies

Targeting: SPTBN2 SCA5

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

711584

Provider

Invitrogen Antibodies

Product name

Spectrin beta-3 Recombinant Polyclonal Antibody (11HCLC)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with Monkey, Cat and Rat.

Antibody clone number

11HCLC

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), HT-29 (Lane 2), HCT 116 (Lane 3), Caco-2 (Lane 4), MCF7 (Lane 5), T-47D (Lane 6), THP-1 (Lane 7) and KARPAS 299 (Lane 8). The blots were probed with Anti-Spectrin Beta 3 Recombinant Rabbit Polyclonal Antibody (Product # 711584, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 268 kDa band corresponding to Spectrin Beta 3 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with overnight wet transfer System. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).