Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-84057 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Calponin 3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: PKYCAAPTEP VIHNGSQGTG TNGSEISDSD YQAEYPDEYH GEYQDDYPRD YQYSDQGIDY
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Calponin 3 by a Calponin 3 polyclonal antibody (Product # PA5-84057). Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Human cell line RT-4 Lane 3: Human cell line U-251 MG.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Calponin 3 in U-2 OS cells using a Calponin 3 polyclonal antibody (Product # PA5-84057). The analysis shows localization to cytosol & actin filaments.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Calponin 3 in human placenta and skeletal muscle tissues using a Calponin 3 polyclonal antibody (Product # PA5-84057). Corresponding RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Calponin 3 in human placenta using a Calponin 3 polyclonal antibody (Product # PA5-84057). The analysis shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Calponin 3 in human skeletal muscle using a Calponin 3 polyclonal antibody (Product # PA5-84057). The analysis shows low expression as expected.