NBP2-22534

antibody from Novus Biologicals

Targeting: CXCL11 b-R1, H174, I-TAC, IP-9, SCYB11, SCYB9B

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-22534

Provider

Novus Biologicals

Product name

Mouse Monoclonal CXCL11/I-TAC Antibody

Antibody type

Monoclonal

Description

Protein G purified.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

200 ug

Concentration

1 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: CXCL11/I-TAC Antibody (22F1) [NBP2-22534] - Analysis of 0.25 ug of recombinant human ITAC and induced HeLa cell culture supernatant onto a 4-20% Tris-HCl polyacrylamide gel.

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

ELISA: CXCL11/I-TAC Antibody (22F1) [NBP2-22534] - Human ITAC was performed by coating a blank 96-well microtiter plate with 100ul per well of Human ITAC monoclonal antibody in duplicate at 1, 3, 4, 5, 7, and 9ug/ml in DPBS and incubating for 12-18 hours at 4C. The plate was aspirated and blocked with 300ul per well of 4% BSA and 5% sucrose in DPBS for 1 hour at room temperature. Human ITAC recombinant protein at 50ul per well was added in duplicate at 4000, 1600, 640, 256, 102.4, 40.96, and 0 pg/ml for 2 hours at room temperature along with 50ul of Human ITAC biotinylated monoclonal antibody in all applicable wells at 0.2ug/ml for 2 hours at room temperature. The plate was washed with ELISA Wash Buffer and incubated with 100ul per well of Streptavidin-HRP in all test wells at 1:80,000 dilution for 1 hour at room temperature and then washed and incubated with 100ul per well of TMB substrate for 30 minutes at room temperature in the dark.