LS-C286015

antibody from LSBio

Targeting: TRIM28 KAP1, PPP1R157, RNF96, TF1B, TIF1B

Western blot (Recommended by provider)

Immunocytochemistry (Enhanced validation)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

LS-C286015

Provider

LSBio

Product name

TRIM28 / KAP1 Antibody (phospho-Ser824) LS-C286015

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Storage

Store at 2-8°C for up to one year.

Immunocytochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of Human Phospho KAP-1 (S824) by Immunocytochemistry. Samples: NBF-fixed, asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) used at a dilution of 1:200 (1 ug/ml). Detection: Red fluorescent Anti-rabbit IgG-DyLight 594 conjugated used at a dilution of 1:100.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of human Phospho KAP-1 (S824) by immunocytochemistry. Samples: NBF-fixed asynchronous HeLa cells grown in chambered microscope slides and treated with etoposide (left) or untreated (right). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) used at a dilution of 1:200 (1µg/ml). Detection: Red fluorescent Anti-rabbit IgG-DyLight® 594 conjugated used at a dilution of 1:100.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of human Phospho KAP-1 (S824) by immunocytochemistry. Samples: FFPE serial sections of asynchronous HeLa cells treated with etoposide (left) and untreated HeLa cells (right). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) used at a dilution of 1:200 (1µg/ml). Detection: DAB

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of Human Phospho KAP-1 (S824) by Immunocytochemistry. Samples: NBF-fixed, asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) used at a dilution of 1:200 (1 ug/ml). Detection: Red fluorescent Anti-rabbit IgG-DyLight 594 conjugated used at a dilution of 1:100.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of human Phospho KAP-1 (S824) by immunocytochemistry. Samples: NBF-fixed asynchronous HeLa cells grown in chambered microscope slides and treated with etoposide (left) or untreated (right). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) used at a dilution of 1:200 (1µg/ml). Detection: Red fluorescent Anti-rabbit IgG-DyLight® 594 conjugated used at a dilution of 1:100.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of human Phospho KAP-1 (S824) by immunocytochemistry. Samples: FFPE serial sections of asynchronous HeLa cells treated with etoposide (left) and untreated HeLa cells (right). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) used at a dilution of 1:200 (1µg/ml). Detection: DAB

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of Human Phospho KAP-1 (S824) by Immunocytochemistry. Samples: NBF-fixed, asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) used at a dilution of 1:200 (1 ug/ml). Detection: Red fluorescent Anti-rabbit IgG-DyLight 594 conjugated used at a dilution of 1:100.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of human Phospho KAP-1 (S824) by immunocytochemistry. Samples: NBF-fixed asynchronous HeLa cells grown in chambered microscope slides and treated with etoposide (left) or untreated (right). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) used at a dilution of 1:200 (1µg/ml). Detection: Red fluorescent Anti-rabbit IgG-DyLight® 594 conjugated used at a dilution of 1:100.