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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Flow cytometry [2]
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Validation data
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- Product number
- TA502615 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA502615, RRID:AB_11128409
- Product name
- BECN1 (Beclin 1) mouse monoclonal antibody, clone OTI2C5 (formerly 2C5)
- Antibody type
- Monoclonal
- Description
- BECN1 (Beclin 1) mouse monoclonal antibody, clone OTI2C5 (formerly 2C5)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- BECN1
- Isotype
- IgG
- Antibody clone number
- OTI2C5
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BECN1 (RC201629, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BECN1.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-BECN1 mouse monoclonal antibody (TA502615).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-BECN1 mouse monoclonal antibody (TA502615) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BECN1(RC201629).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-BECN1 antibody(TA502615),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC201629 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-BECN1 antibody(TA502615), and then analyzed by flow cytometry.
- Validation comment
- FC
