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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [2]
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Validation data
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- Product number
- GTX132407 - Provider product page
- Provider
- GeneTex
- Product name
- Ras (G12D Mutant Specific) antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
- Storage
- Keep as concentrated solution. Aliquot and store at -20?C or below. Avoid multiple freeze-thaw cycles.
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Ras (G12D Mutant specific) antibody detects Ras (G12D Mutant specific) protein by western blot analysis. Non-transfected (-) and Ras (G12D Mutant specific)-transfected (+) 293T whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Ras (G12D Mutant specific) antibody (GTX132407) diluted at 1:5000.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Ras (G12D Mutant Specific) antibody (GTX132407) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Ras (G12D Mutant specific) antibody (GTX132407) diluted at 1:5000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Ras (G12D Mutant Specific) antibody detects Ras (G12D Mutant Specific) protein in colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded colon cancer. Ras (G12D Mutant Specific) antibody (GTX132407) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Ras (G12D Mutant Specific) antibody detects Ras (G12D Mutant Specific) protein in colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded colon cancer. Ras (G12D Mutant Specific) antibody (GTX132407) diluted at 1:1000.
