PA1-083

antibody from Invitrogen Antibodies

Targeting: XPO1 CRM-1, CRM1, emb

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-083

Provider

Invitrogen Antibodies

Product name

CRM1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA1-083 detects CRM1 (chromosome region maintenance 1) from bovine retinal extract, human and mouse samples.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot detection of CRM1 from bovine retinal extract using Product # PA1-083.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Exportin 1 was performed by loading 25 µg of SK-BR-3 (lane 1), Hela (lane 2) and NIH-3T3 (lane 3) onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with an Exportin 1 polyclonal antibody (Product # PA1-083) at a dilution of 1:1000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~130 kDa.