56-1278-42

antibody from Invitrogen Antibodies

Targeting: IL7R CD127, IL7RA

Provider product page for 56-1278-42

Flow cytometry

Other assay

Antibody data

Antibody Data
Antigen structure
References [11]
Comments [0]
Validations
Flow cytometry [1]
Other assay [15]

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Product number: 56-1278-42 - Provider product page
Provider: Invitrogen Antibodies
Product name: CD127 Monoclonal Antibody (eBioRDR5), Alexa Fluor™ 700, eBioscience™
Antibody type: Monoclonal
Antigen: Other
Description: The eBioRDR5 monoclonal antibody reacts with human CD127 (Interleukin-7 Receptor alpha). CD127 complexes with CD132, also known as the common gamma chain (gamma c), to form the multi-functional IL-7 receptor (IL-7R). CD127 is a type I glycoprotein with a molecular weight of 75-80 kDa and is expressed by immature B cells through the early pre-B stage, by thymocytes during several stages of their development, and on most mature T cells, with transient down-regulation upon activation. Binding of IL-7 results in signal transduction which occurs through several tyrosine kinase pathways including the Jak/STAT pathway. IL-7 is indispensible for the development of lymphocytes, and the control of homeostatic proliferation of T-cells in the periphery. In addition, IL-7R signaling is know to be involved in the regulation of T cell receptor (TCR) locus rearrangement in gamma delta T cells.
Conjugate: Near infrared dye
Antibody clone number: eBioRDR5
Concentration: 5 µL/Test

IL7R protein structure - 56-1278-42 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL
Conjugate: Near infrared dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL
Conjugate: Near infrared dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL
Conjugate: Near infrared dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL
Conjugate: Near infrared dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL
Conjugate: Near infrared dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL
Conjugate: Near infrared dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL
Conjugate: Near infrared dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL
Conjugate: Near infrared dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL
Conjugate: Near infrared dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: FIGURE 1 Identification of Tscm CD4 + CD45RA + CD45RO - CD62L + CCR7 + CD127 + CD27 + CD28 + CD95 + CD122 + T (Tscm) cell in human blood and lymph nodes. PBMCs were isolated from the blood of non-small cell lung cancer (NSCLC) patients (n=15) (NSCLC-PBMC) and healthy donors (n=11) (HD-PBMC); lymphocytes were isolated from the tumor-infiltrated lymph node of NSCLC patients who were collected blood at same time (n=7) (NSCLC-Ly); lymphocytes were isolated from the healthy lymph node of non lung cancer patients (n=7) (Normal-Ly), analyzed by flow cytometry. A, Representative flow cytometric analyses of CD4 + CD45RA + CD45RO - CD62L + CCR7 + CD127 + CD27 + CD28 + CD95 + CD122 + T cells, indicating Tscm cells. B, The frequency of the CD4 + Tscm cells in the HD-PBMC, NSCLC-PBMC, Normal-Ly, NSCLC-Ly. The events of CD4 + Tscm cells in the blood and lymph node from NSCLC patients and healthy donors, expressed as the mean+-SEM. C, The frequency of the CD8 + Tscm cells in the HD-PBMC, NSCLC-PBMC, Normal-Ly, NSCLC-Ly, expressed as the mean+-SEM. D, The events of Tscm of CD4 + and CD8 + cells in the blood and the lymph node from NSCLC patients and healthy donors. HD indicates healthy donors; IFN, interferon; PBMC, peripheral blood mononuclear cells; Tscm cell, stem cell-like memory T cell. * P
Conjugate: Near infrared dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FIGURE 3 CD4 + CD45RA + CD45RO - CD95 - CD122 + CD127 + T cell population displays different phenotypes in human blood and lymph nodes. A, Flow cytometry plots showing IFN-gamma expression in the CD4 + CD45RA + /CD45RO - CD95 - CD122 + CD127 + T cells from the blood and lymph node of the non-small cell lung cancer (NSCLC) patients and healthy donors. B, The mean frequency (+-SEM) of the events of CD4 + CD45RA + /CD45RO - CD95 - CD122 + CD127 + T cells. C, The mean IFN-gamma production (+-SEM) of the CD4 + CD45RA + /CD45RO - CD95 - CD122 + CD127 + T cells. HD indicates healthy donors; IFN, interferon; PBMC, peripheral blood mononuclear cells; PMA, phorbol 12-myristate13-acetate. * P
Conjugate: Near infrared dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 6 a Representative flow cytometry data, demonstrating the gating strategy used on PBMC for Treg identification and analysis. FSC-area vs. FSC-height was used for doublet discrimination. A ""dump"" channel was used to gate out dead cells (LIVE/DEAD fixable viability stain), CD14 + monocytes and CD19 + B cells, and lymphocytes were subsequently selected by FSC vs. SSC. CD4 + T cells were gated on the basis of CD4 vs. CD3 staining, then examined for expression of Ki67 and ICOS. Tregs were identified within the CD4 + T cell population as CD25 hi CD127 lo and Foxp3 + . b Longitudinal empirical data, linear mixed models and estimated means (left, centre and right-hand panels respectively) for Ki67+ and ICOS+ expression on CD4+ T cells, and the Treg proportion of CD4 cells ( P -values: *
Conjugate: Near infrared dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 6 In vivo knockdown of PITPNM3 in CD4 + T cells reverses immunosuppression and inhibits tumor progression in humanized mice. (A) Humanized mice bearing palpable MDA-MB-231 orthotopic xenografts were intraperitoneally injected daily for 14 days with PBS, 1 nmol CD4-aptamer-control siRNA (AsiC-con) or CD4-aptamer-siRNA targeting PITPNM3 (sequence in A , AsiC-PI) to assess the role of PITPNM3 in TI Tregs, and other T cells and tumor control. Experimental schematic is provided in Supplementary information, Figure S9A . (B) Representative immunoblots showing selective knockdown of PITPNM3 protein in PB CD4 + T cells, but not tumor xenografts ( n = 3). (C) PITPNM3 knockdown did not affect the distribution of human CD45 + hematopoietic cells, CD4 + and CD8 + T cells, and CD14 + monocytes in the peripheral blood of humanized mice. Representative flow plots are shown ( n = 3). (D , E) Effect of PITPNM3 knockdown on TI naive CD4 + , Tregs and CD8 + T cell numbers, and apoptosis by TUNEL assay in xenografts. D shows representative immunofluorescence microscopy images. Top row indicates CD4 + naive T cells by arrows; the second row indicates CD4 + CD45RO + Foxp3 - CD4 + memory T cells (yellow arrows) and Foxp3 + Tregs (white arrows). Scale bar, 50 mum. E shows number of cells of each subtype/high power field in eight mice ( ** P < 0.01, *** P < 0.001 compared to PBS group by Student's t -test). (F) Flow cytometry analysis of gated human CD3 + CD4 + cells isolated from xenogra
Conjugate: Near infrared dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 4. miR-21 Promotes Effector Cell Differentiation through AP-1 Activation (A) Naive CD4 + T cells were transfected with either scrambled control or miR-21-blocking locked nucleic acid (LNA21). After 48 hr, PDCD4 and b-actin expression were assessed by western blot. Representative blots and mean normalized intensities from four experiments are shown (mean +- SEM, two-tailed paired t test). (B) Naive CD4 + T cells were activated with anti-CD3 and anti-CD28 beads and transduced with a lentiviral vector expressing scrambled control or anti-miR-21. The representative histogram shows phosphorylated JNK in GFP + cells on day 3. The filled gray histogram represents unstimulated naive CD4 + T cells. Results from 7 experiments are expressed relative to the geometric mean fluorescence intensity (MFI) of controls (mean +- SEM, two-tailed paired t test). (C) Naive CD4 + T cells were activated and transduced as described in (B) and co-transfected with the AP-1 luciferase reporter plasmid and the Renilla luciferase control construct. On day 3, the activity of AP-1 firefly luciferase was measured and normalized to that of Renilla luciferase (n = 4, mean +- SEM, two-tailed paired t test). (D-F) Naive CD4 + T cells isolated from 20- to 35-year-old and 65- to 85-year-old individuals were activated with beads coated with anti-CD3 and anti-CD28 antibodies. (D) PDCD4 expression was quantified by RT-PCR on day 3 and day 5. Results are normalized to ACTB and presented relative to
Conjugate: Near infrared dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 6 Greater numbers of interleukin (IL)-7Ralpha high effector memory (EM) CD8 + T cells than IL-7Ralpha low EM CD8 + T cells in the skin. (A) Immunofluorescence staining (40x) of CD8 + T cells (green) from non-lesional (healthy, HC) or lesional atopic dermatitis skin. IL-7Ralpha + CD8 + T cells (upper panel) representing IL-7Ralpha high EM CD8 + T cells were stained with antibodies (Abs) to IL-7Ralpha (red); perforin + CD8 + T cells (lower panel) representing IL-7Ralpha low EM CD8 + T cells, were stained with Abs to perforin (red). The image of the box was magnified twice and placed to the right of each image. (B) A quantitative measurement of IL-7Ralpha + and perforin + CD8 + T cells (frequency and number per tissue) in panel (A) , representing IL-7Ralpha high and IL-7Ralpha low EM CD8 + T cells, respectively. Four images per slide were evaluated for quantification. Data are representative of four independent experiments. Bars represent the mean, and p -values were obtained using the Wilcoxon matched pairs test (for comparing frequency and number between the two CD8 + T cell subsets) and Mann-Whitney U test (for comparing IL-7Ralpha + /perforin + ratio between HC and dermatitis).
Conjugate: Near infrared dye