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- Western blot [3]
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- Product number
- GTX48714 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX48714, RRID:AB_11172726
- Product name
- IL7 Receptor alpha (phospho Tyr449) antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western Blot of Rabbit anti- IL-7-Receptor-alpha-chain-pY449 antibody (GTX48714). Load: thymocyte D1 cells treated with or without IL-7 (50 ng/ml) for 20 min. Primary antibody: IL7pY449 antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 55 kDa, ~64 kDa for IL7pY449. Other band(s): unspecifics.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of Rabbit anti- IL-7-Receptor-alpha-chain-pY449 antibody. Lane 1: thymocyte D1 cells. Lane 2: thymocyte D1 cells treated with IL7 (50 ng/ml). IP: with anti-Phosphotyrosine conjugated to Protein G agarose. Primary antibody: IL7pY449 antibody at 1:10000 for overnight at 4¢XC. Secondary antibody: HRP-conjugated goat anti-rabbit antibody at 1:10,000 for 45 min at RT and ECL detection. Block: 5% BLOTTO overnight at 4¢XC. Predicted/Observed size: 55 kDa, ~64 kDa for IL7pY449. Other band(s): unspecifics.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western Blot of Rabbit anti- IL-7-Receptor-alpha-chain-pY449 antibody (GTX48714). Load: thymocyte D1 cells treated with or without IL-7 (50 ng/ml) for 20 min. Primary antibody: IL7pY449 antibody at 1:1000 for overnight at 4¢XC. Secondary antibody: IRDye800? rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4¢XC. Predicted/Observed size: 55 kDa, ~64 kDa for IL7pY449. Other band(s): unspecifics.
