Explore
Validate
LearnRP1086
antibody from Boster Biological Technology
Targeting: FOSB
AP-1, DKFZp686C0818, G0S3, GOS3, GOSB, MGC42291
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- RP1086 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Fos B/FOSB Antibody
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for G0S3/FOSB detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. G0S3/FOSB information: Molecular Weight: 35928 MW; Subcellular Localization: Nucleus.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
No comments: Submit comment
Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of FOSB using anti-FOSB antibody (RP1086). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: mouse thymus tissue lysates, Lane 3: mouse ovary tissue lysates, Lane 4: mouse spleen tissue lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse RAW246.7 whole cell lysates, Lane 7: human HL-60 whole cell lysates, Lane 8: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOSB antigen affinity purified polyclonal antibody (Catalog # RP1086) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOSB at approximately 35KD. The expected band size for FOSB is at 35KD.
- Additional image
