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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 17-8849-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD221 (IGF1R) Monoclonal Antibody (1H7), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This 1H7 monoclonal antibody reacts with human insulin-like growth factor-1 receptor (IGF-1R), also known as CD221. This receptor is a cell surface-expressed glycoprotein composed of two extracellular alpha subunits and two transmembrane beta subunits that possess tyrosine kinase activity. Expressed on nearly all cell types, this receptor binds IGF-I and IGF-II, as well as insulin. Ligand binding leads to activation of the PI3K/Akt and MAPK pathways, which mediate cell proliferation and survival. Many tumors and transformed cells display altered IGF-1R expression.
- Antibody clone number
- 1H7
- Concentration
- 5 µL/Test
Submitted references Effects of IGF-1 on Proliferation, Angiogenesis, Tumor Stem Cell Populations and Activation of AKT and Hedgehog Pathways in Oral Squamous Cell Carcinoma.
Functional and differential proteomic analyses to identify platelet derived factors affecting ex vivo expansion of mesenchymal stromal cells.
Ferreira Mendes JM, de Faro Valverde L, Torres Andion Vidal M, Paredes BD, Coelho P, Allahdadi KJ, Coletta RD, Souza BSF, Rocha CAG
International journal of molecular sciences 2020 Sep 5;21(18)
International journal of molecular sciences 2020 Sep 5;21(18)
Functional and differential proteomic analyses to identify platelet derived factors affecting ex vivo expansion of mesenchymal stromal cells.
Kinzebach S, Dietz L, Klüter H, Thierse HJ, Bieback K
BMC cell biology 2013 Oct 30;14:48
BMC cell biology 2013 Oct 30;14:48
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of the HeLa cell line with Mouse IgG1 kappa Isotype Control APC (Product # 17-4714-81) (blue histogram) or Anti-Human CD221 (Insulin-like Growth Factor-1 Receptor) APC (purple histogram). Total viable cells were used for analysis.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 Evaluation of IGF-1, IGF1R and HH pathway expression in fibroblasts and oral squamous carcinoma cells. ( A ) Representative histogram of IGF1R (CD221, dark histogram) expression in SCC-4 cells assessed by flow cytometry. Unstained cells were used as controls (lighter histogram). ( B ) IGF1 mRNA expression in SCC-4 and MF1 cells, evaluated by RT-qPCR. ( C ) Evaluation of HH pathway component expression in MF1 by immunocytochemistry. Magnification: 400x.
