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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB5451 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal IL-5 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects rat IL-5 in direct ELISAs. In direct ELISAs, 50% cross-reactivity with mouse IL-5 is observed and no cross-reactivity with human, equine, canine, feline, or rhesus monkey IL-5 is observed.
- Reactivity
- Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Cell Proliferation Induced by IL-5 and Neutralization by Rat IL-5 Antibody. Recombinant Rat IL-5 (Catalog # 545-RL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-5 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat IL-5 Monoclonal Antibody (Catalog # MAB5451). The ND50 is typically 0.02-0.08 µg/mL.
