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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB205-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-5 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human IL-5 in direct ELISAs. In direct ELISAs, approximately 3% cross-reactivity with recombinant mouse IL-5 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P05113- Isotype
- IgG
- Antibody clone number
- 14611
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Inhibition of interleukin-5 induced false positive anti-drug antibody responses against mepolizumab through the use of a competitive blocking antibody.
Human CD8+ T-cells recognizing peptides from Mycobacterium tuberculosis (Mtb) presented by HLA-E have an unorthodox Th2-like, multifunctional, Mtb inhibitory phenotype and represent a novel human T-cell subset.
Regulation of the interleukin-5 receptor alpha-subunit on peripheral blood eosinophils from healthy subjects.
Liao K, Meyer E, Lee TN, Loercher A, Sikkema D
Journal of immunological methods 2017 Feb;441:15-23
Journal of immunological methods 2017 Feb;441:15-23
Human CD8+ T-cells recognizing peptides from Mycobacterium tuberculosis (Mtb) presented by HLA-E have an unorthodox Th2-like, multifunctional, Mtb inhibitory phenotype and represent a novel human T-cell subset.
van Meijgaarden KE, Haks MC, Caccamo N, Dieli F, Ottenhoff TH, Joosten SA
PLoS pathogens 2015 Mar;11(3):e1004671
PLoS pathogens 2015 Mar;11(3):e1004671
Regulation of the interleukin-5 receptor alpha-subunit on peripheral blood eosinophils from healthy subjects.
Hellman C, Halldén G, Hylander B, Lundahl J
Clinical and experimental immunology 2003 Jan;131(1):75-81
Clinical and experimental immunology 2003 Jan;131(1):75-81
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by IL-5 and Neutralization by Human IL-5 Antibody. Recombinant Human IL-5 (Catalog # 205-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL-5 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-5 Monoclonal Antibody (Catalog # MAB205). The ND50 is typically 0.01-0.03 µg/mL.
