Explore
Validate
Learn
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- M551 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-5 Monoclonal Antibody (5A10)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- M551 targets IL-5 in ELISA, and WB applications and shows reactivity with Human samples.
- Antibody clone number
- 5A10
- Concentration
- 1.0 mg/mL
Submitted references Natural regulatory T cells: number and function are normal in the majority of patients with lupus nephritis.
Insulin-like growth factor-I stimulates IL-10 production in human T cells.
Yates J, Whittington A, Mitchell P, Lechler RI, Lightstone L, Lombardi G
Clinical and experimental immunology 2008 Jul;153(1):44-55
Clinical and experimental immunology 2008 Jul;153(1):44-55
Insulin-like growth factor-I stimulates IL-10 production in human T cells.
Kooijman R, Coppens A
Journal of leukocyte biology 2004 Oct;76(4):862-7
Journal of leukocyte biology 2004 Oct;76(4):862-7
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA analysis of human IL-5 was performed by coating wells of a 96-well plate with 110 µL of a human IL-5 monoclonal antibody (Product # M551) in duplicate at 1, 2, 4, 5, 7, and 9 µg/mL for at least 12 hours. The plate was blocked with a proprietary blocking solution for 1-4 hours at room temperature, and 50 µL of mouse IL-5 recombinant protein (Product # SMIL5) was added in replicates of twelve at 400, 160, 64, 25.6, 10.24, and 0pg/mL for 1 hour at room temperature. The plate was washed and incubated 50 µL of a biotinylated IL-5 monoclonal antibody (Product # M550B) was added to all test wells at 0.5 µg/mL for 1 hour at room temperature. The plate was washed, then incubated with 100 µL per well of Streptavidin-HRP (Product # N504) at a 1:10,000 dilution for 30 minutes at room temperature. Detection was performed with 100 µL per well of Ultra TMB substrate (Product # 34028) for 30 minutes at room temperature in the dark. The plate was then stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550 nm.
