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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF-205-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-5 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human IL-5 in direct ELISAs and Western blots. In Western blots, approximately 25% cross-reactivity with recombinant mouse IL-5 is observed. Neutralizes the biological activity of recombinant IL-5 and will also neutralize the biological activity of recombinant mouse IL-5 at a 10-fold higher IgG concentration.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P05113- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Secretory IgA induces antigen-independent eosinophil survival and cytokine production without inducing effector functions.
Development and validation of a kinetic assay for analysis of anti-human interleukin-5 monoclonal antibody (SCH 55700) and human interleukin-5 interactions using surface plasmon resonance.
Bleomycin stimulates lung fibroblast and epithelial cell lines to release eosinophil chemotactic activity.
Bartemes KR, Cooper KM, Drain KL, Kita H
The Journal of allergy and clinical immunology 2005 Oct;116(4):827-35
The Journal of allergy and clinical immunology 2005 Oct;116(4):827-35
Development and validation of a kinetic assay for analysis of anti-human interleukin-5 monoclonal antibody (SCH 55700) and human interleukin-5 interactions using surface plasmon resonance.
DiGiacomo RA, Xie L, Cullen C, Indelicato SR
Analytical biochemistry 2004 Apr 15;327(2):165-75
Analytical biochemistry 2004 Apr 15;327(2):165-75
Bleomycin stimulates lung fibroblast and epithelial cell lines to release eosinophil chemotactic activity.
Sato E, Koyama S, Robbins RA
The European respiratory journal 2000 Nov;16(5):951-8
The European respiratory journal 2000 Nov;16(5):951-8
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by IL-5 and Neutralization by Human IL-5 Antibody. Recombinant Human IL-5 (Catalog # 205-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-5 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-205-NA). The ND50 is typically 0.3-0.9 µg/mL.
