500337

antibody from BioLegend

Targeting: IL2 IL-2, TCGF

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

500337

Provider

BioLegend

Proper citation

BioLegend Cat#500337, RRID:AB_2562629

Product name

Brilliant Violet 510™ anti-human IL-2

Antibody type

Monoclonal

Antigen

E. coli - expressed recombinant human IL-2

Description

IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells, promoting proliferation and maturation. Additionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes.

Reactivity

Human

Host

Rat

Conjugate

Yellow dye

Antibody clone number

MQ1-17H12

Vial size

25 tests

Storage

2°C-8°C

Handling

Ambient RT

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

PMA + Ionomycin-stimulated (6 hours) human peripheral blood lymphocytes (in the presence of monensin) were surface stained with CD3 APC, fixed, permeabilized, and then stained with IL-2 (clone MQ1-17H12) Brilliant Violet 510™ (top) or rat IgG2a, κ Brilliant Violet 510™ isotype control (bottom).

Conjugate

Green dye