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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF579 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IL-2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detect cotton rat IL-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant rat IL-2 and recombinant mouse IL-2 is observed and less than 5% cross-reactivity with recombinant human IL-2 and recombinant porcine IL-2 is observed.
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Cell Proliferation Induced by IL-2 and Neutralization by Cotton Rat IL-2 Antibody. Recombinant Cotton Rat IL-2 (Cys146Tyr) (Catalog # 579-R2) stimulates proliferation in the CTLL-2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Cotton Rat IL-2 (Cys146Tyr) (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat IL-2 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF579). The ND50 is typically 0.1-0.4 µg/mL.
