500325

antibody from BioLegend

Targeting: IL2 IL-2, TCGF

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

500325

Provider

BioLegend

Proper citation

BioLegend Cat#500325, RRID:AB_2125594

Product name

PE/Cy7 anti-human IL-2

Antibody type

Monoclonal

Antigen

E. coli - expressed recombinant human IL-2

Description

IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells, promoting proliferation and maturation. Additionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes.

Reactivity

Human, Simian

Host

Rat

Conjugate

Infrared dye

Isotype

IgG

Vial size

25 tests

Storage

The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References

Submitted references

Primary human immunodeficiency virus type 1-specific CD8+ T-cell responses induced by myeloid dendritic cells.

Colleton BA, Huang XL, Melhem NM, Fan Z, Borowski L, Rappocciolo G, Rinaldo CR
Journal of virology 2009 Jun;83(12):6288-99

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Regulation of CD8+ T lymphocyte effector function and macrophage inflammatory cytokine production by retinoic acid receptor gamma.

Dzhagalov I, Chambon P, He YW
Journal of immunology (Baltimore, Md. : 1950) 2007 Feb 15;178(4):2113-21

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

PMA+ionomycin-stimulated (6 hours) peripheral blood lymphocytes surface stained with CD3 FITC, then intracellularly stained with MQ1-17H12 PE/Cy7 (top) or rat IgG2a, k PE/Cy7 isotype control (bottom)

Conjugate

Near infrared dye