Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- TA300051 - Provider product page
- Provider
- OriGene
- Product name
- PIM2 Antibody (C-term ) Rabbit Polyclonal Antibody (Pab)
- Antibody type
- Polyclonal
- Description
- PIM2 Antibody (C-term ) Rabbit Polyclonal Antibody (Pab)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- PIM2
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 0.25 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of anti-PIM2 Antibody (C-term) (Cat.#TA300051) in Hela cell line lysates (35ug/lane). PIM2 (arrow) was detected using the purified Pab.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of PIM2 (arrow) using rabbit polyclonal PIM2 Antibody (D292) (Cat.#TA300051). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PIM2 gene.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- PIM proteins were immunoprecipitated from MV4;11 cells and the agarose-protein A-immunoprecipitate complex was tested for its ability to phosphorylate BAD in vitro in the presence or absence of K00135. Phosphorylation of BAD (both on Ser112 and Ser136) was abrogated on addition of the compound. Beads alone (without anti-PIM antibodies) as a negative control.
- Validation comment
- IP
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
- Validation comment
- IHC