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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF959 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Erythropoietin/EPO Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human, mouse, and rat Erythropoietin/EPO in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse Thymopoietin (Tpo) is observed.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Zinc Supplementation Stimulates Red Blood Cell Formation in Rats.
Suppression of hepcidin during anemia requires erythropoietic activity.
Chen YH, Feng HL, Jeng SS
International journal of molecular sciences 2018 Sep 18;19(9)
International journal of molecular sciences 2018 Sep 18;19(9)
Suppression of hepcidin during anemia requires erythropoietic activity.
Pak M, Lopez MA, Gabayan V, Ganz T, Rivera S
Blood 2006 Dec 1;108(12):3730-5
Blood 2006 Dec 1;108(12):3730-5
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Cell Proliferation Induced by Erythropoietin and Neutralization by Mouse Erythropoietin Antibody. Recombinant Mouse Erythropoietin (Catalog # 959-ME) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Erythropoietin (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse/Rat Erythropoietin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF959). The ND50 is typically 0.25-1.25 µg/mL.
